别名:
人IgE全长蛋白质
研究领域:
免疫学
详述
抗体应用:
未定义
蛋白长度Full length protein
描述
性质Natural
来源Native
氨基酸序列
种属Human
额外的序列信息Observed band of ~185kDa on SDS-PAGE
技术指标
Our Abpromise guarantee covers the use of ab65866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用
SDS-PAGE
ELISA
Immunocytochemistry
内毒素水平< 25.000 Eu/mg
纯度> 90 % by SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
形式Liquid
补充说明
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.
Less batch-to-batch variation
Higher degree of purity (>90%)
A more uniform product (monoclonal and not polyclonal)
IgE has been used in a variety of cell stimulation assays, including the following cell types:
Human airway smooth muscle (HASM) cells
mast cells (MC)
Human bronchial/tracheal smooth muscle (B/TSM) cells
acute myelogenous leukemia (AML) blasts
Human neutrophils
This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.
1 mg of ab65866 is 380,000± 50,000 IU/ml.
浓度100 |
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