¸ÅÊö
Õâ¸öAbraxisÖùæßÔ嶾ËØELISA¼ì²âÊÔ¼ÁºÐ£¬ÊÊÓÃÓÚ¶¨Á¿¼ì²âË®ÑùÖÐÖùæßÔ嶾Ëصĺ¬Á¿¡£ÔÚÑùÆ·´¦ÀíµÄʱºò²»ÐèҪŨËõ¡£Èç¹û±ØÒªµÄ»°ÑôÐÔÑùÆ·¿ÉÒÔͨ¹ýHPLC»òÆäËûµÄ´«Í³µÄ·½·¨À´ÑéÖ¤¡£
°²È«ÐÔ˵Ã÷
µ×ÎïÈÜÒº°üº¬ÓÐTMB£¬ÖÕÖ¹Òºº¬ÓÐÏ¡ÁòËá¡£Òª±ÜÃâƤ·ôºÍµ×ÎïÈÜÒºÓëÖÕÖ¹Òº½Ó´¥¡£Èç¹û²»Ð¡ÐĽӴ¥µÄ»°ÇëÓÃÇåË®³åÏ´¡£
´¢´æºÍÎȶ¨ÐÔ
Õâ¸öAbraxisÖùæßÔ嶾ËØELISA¼ì²âÊÔ¼ÁºÐÓ¦¸ÃÖÃÓÚ4¨C8¡ãC±£´æ¡£ÔÚʹÓÃÇ°ÒªÌáÇ°ÄóöÀ´»Ø¸´µ½ÊÒΣ¨20-25¡ãC£©¡£ÔÚÓÐЧÆÚÄÚ¶¼¿ÉÒÔʹÓá£
ÔÀí
Õâ¸öAbraxisÖùæßÔ嶾Ëؼì²âÊÔ¼ÁºÐµÄÔÀíÊÇÖ±½Ó¾ºÕùøÁªÃâÒß·´Ó¦¡£Í¨¹ýÒ»ÖÖÌØÒìÐÔ¿¹ÌåÀ´Ê¶±ð¼ì²âÖùæßÔ嶾ËØ¡£µ±ÑùÆ·Öл¹ÓÐÖùæßÔ嶾Ëؼ°ÆäÀàËÆÎïʱ£¬ÄÇôËüÃǽ«»áºÍÖùæßÔ嶾ËØ-HRP¾ºÕùºÍÈÜÒºÖеÄ΢ÄÒÔåËØ¿¹Ìå½áºÏ¡£Î¢ÄÒÔåËØ¿¹ÌåÓë°ü±»ÔÚ΢¿×°åµ×²¿µÄÑò¿¹Êó¶þ¿¹½áºÏ¡£¾¹ýÒ»¸öÏ´µÓ²½Öèºó¼ÓÈëÎÞÉ«µ×Î²úÉúÁËÒ»¸öÑÕÉ«·´Ó¦¡£¼ÓÈë·´Ó¦ÖÕÖ¹ÒººóʹÑÕÉ«ÓÉÀ¶É«±äΪ»ÆÉ«£»ÔÚ450nm²¨³¤½øÐмì²â£¬ÑùÆ·ÖеÄ΢ÄÒÔåËØŨ¶ÈÓëÎüÊÕ¹âÇ¿¶È³É·´±È¡£
ÊÔ¼ÁºÐµÄ¾ÖÏÞÐԺͿÉÄܵĽ»²æ·´Ó¦
¶ÔÔÚË®ÑùÖо³£³öÏֵĶàÖÖÓлúÎïºÍÎÞ»úÎïÒѽü×÷¹ý¼ì²â¶Ô´ËÊÔ¼ÁºÐ²¢ÎÞ¸ÉÈÅ¡£ÓÉÓÚ»¯ºÏÎïµÄÒ×±äÖÊÐÔÓÉ»ùÖÊ·´Ó¦ÒýÆðµÄ¸ÉÈÅÊDz»¿ÉÄÜÍêÈ«±ÜÃâµÄ¡£ÒÔÏÂÎïÖʵÄŨ¶ÈÔÚ²»¸ßÓÚ10,000 ppmʱ¶ÔÊÔ¼ÁºÐ¼ì²â½á¹ûûÓÐÓ°Ï죺Ñõ»¯ÂÁ¡¢ÂÈ»¯¸Æ¡¢ÁòËá¸Æ¡¢ÁòËáÃÌ¡¢ÁòËáþ¡¢ÂÈ»¯Ã¾¡¢ÂÈ»¯ÄÆ¡¢Á×ËáÑΡ¢Áò´úÁòËáÄÆ¡£ÒÔÏÂÎïÖʵÄŨ¶ÈÔÚ²»¸ßÓÚ1,000 ppmʱ¶ÔÊÔ¼ÁºÐ¼ì²â½á¹ûûÓÐÓ°Ï죺ÏõËáÄÆ¡¢ÁòËáп£»¸¯Ö³ËáСÓÚ100 ppm¶Ô¼ì²â½á¹ûûÓÐÓ°Ï죻ÂÈ»¯ÍСÓÚ10 ppm¶Ô¼ì²â½á¹ûûÓÐÓ°Ïì¡£²Ù×÷²»µ±¿ÉÄܵ¼Ö¼ì²â½á¹û´íÎ󣬱ÈÈ磺ÊÔ¼ÁºÐ±£´æÌõ¼þ²»¶Ô¡¢´íÎóµÄ¼ÓҺ˳Ðò¡¢ÊÔ¼ÁµÄÁ¿Ã»ÓмÓ׼ȷ¡¢·õÓýµÄʱ¼äÌ«³¤»òÌ«¶Ì¡¢·õÓýµÄζÈÌ«¸ß»òÌ«µÍ¡£²Ù×÷¹ý³Ì²»Ò×ÔÚÇ¿¹âϽøÐС£ºÍÆäËü¼ì²â·½·¨Ò»Ñù¶ÔÑôÐÔ½á¹ûÒªÇóͨ¹ýÆäËü´«Í³µÄ·½·¨À´ÑéÖ¤¡£
ÊÔ¼ÁºÐ×é³É
1¡¢96¿×ø±ê°å£º1¿é£¨12¡Á8Ìõ£©£¬°ü±»ÓÐÑò¿¹Íöþ¿¹¡£
2¡¢±ê×¼ÈÜÒººÍ¶ÔÕÕ:±ê×¼7Æ¿£¬Å¨¶È·Ö±ðΪ0, 0.05, 0.10, 0.25, 0.50, 1.0, 2.0 ng/mL£¬¶ÔÕÕ1ƿŨ¶ÈΪ0.75 ng/mL
3¡¢¿¹ÌåÈÜÒº£¨Íÿ¹ÖùæßÔ嶾ËØ¿¹Ì壩£º6ml
4¡¢ÖùæßÔ嶾ËØ-HRPø±ê¼ÇÎ6ml
5¡¢Áã±ê×¼/Ï¡ÊÍÒº25mlÓÃÓÚÏ¡ÊÍÑùÆ·£¨ÑùƷŨ¶È´óÓÚ2 ppbʱ£©
6¡¢Ï´Òº5X Concentrate, 100 mL
5¡¢µ×ÎïÏÔÉ«Òº£¨TMB£©12ml
6¡¢ÖÕÖ¹Òº£º2Æ¿¡¢Ã¿Æ¿6ml
7¡¢Ê¹ÓÃ˵Ã÷Êé |
![](http://img.qy6.com.cn/new3/reagen16/1307953569.jpg) |
|